Journal: STAR Protocols

Article Title: Protocol for parotidectomy and saliva analysis in mice

doi: 10.1016/j.xpro.2021.101048

Figure Lengend Snippet:

Article Snippet: Sterile Collection Swab, 20mm break point , ZYMO , Cat#: C1052-50.

Techniques: Recombinant

Journal: ACS Omega

Article Title: CHAMP: A Centrifugal Microfluidics-Based CRISPR/Cas12b-Combined Real-Time LAMP One-Pot Method for Mycoplasma pneumoniae Infection Diagnosis

doi: 10.1021/acsomega.4c05489

Figure Lengend Snippet: Study participants and illustration of included and excluded. (a) Participants included individuals with respiratory infectious diseases (including M. pneumoniae and other respiratory diseases) and healthy donors. DNA extractions from clinical nasopharyngeal swab specimens were used to evaluate the CHAMP method’s performance. The evaluation has three groups: the M. pneumoniae positive group, the infection caused by other pathogens group, and the healthy participants group. (b) Age distribution among different groups. The error bars represent the median with the interquartile range. (c) The sex distribution of study groups.

Article Snippet: Clinical nasopharyngeal swab collection tubes (model: X105) and cell preservation solution used for specimen collection and storage, consisting of tris hydrochloride, guanidine thiocyanate, sodium hydroxide, ethylenediaminetetraacetic acid disodium salt dehydrate, RNase inhibitor, ProClin 300, and phenol red, were purchased from Sansure Biotech Inc. (China). dTTP, dATP, dCTP, and dGTP reagents were purchased from BBI Life Sciences Corporation (Shanghai, China).

Techniques: Infection

Journal: ACS Omega

Article Title: CHAMP: A Centrifugal Microfluidics-Based CRISPR/Cas12b-Combined Real-Time LAMP One-Pot Method for Mycoplasma pneumoniae Infection Diagnosis

doi: 10.1021/acsomega.4c05489

Figure Lengend Snippet: Positive and negative predictive values, sensitivity, and specificity of this CHAMP method for diagnosing M. pneumonia in nasopharyngeal Samples

Article Snippet: Clinical nasopharyngeal swab collection tubes (model: X105) and cell preservation solution used for specimen collection and storage, consisting of tris hydrochloride, guanidine thiocyanate, sodium hydroxide, ethylenediaminetetraacetic acid disodium salt dehydrate, RNase inhibitor, ProClin 300, and phenol red, were purchased from Sansure Biotech Inc. (China). dTTP, dATP, dCTP, and dGTP reagents were purchased from BBI Life Sciences Corporation (Shanghai, China).

Techniques: CRISPR